世界地质 2017, 36(2) 632-642 DOI:   10.3969/j.issn.1004-5589.2017.02.031  ISSN: 1004-5589 CN: 22-1111/P

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克隆文库
多样性
细菌群落结构
长白山
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PubMed
长白山小天池和长白瀑布水体典型样本的细菌多样性比较
张修源1, 苗雨雁2,3, 顾政权4, 刘风香5
1. 南京理工大学机械工程学院, 南京 210094;
2. 中国地质大学生物地质与环境地质国家重点实验室, 武汉 430074;
3. 北京自然博物馆, 北京 100050;
4. 中国科学院青藏高原研究所, 北京 100101;
5. 中国地质博物馆, 北京 100034
摘要

水体中微生物对环境的改变有着敏感的响应。16S rRNA基因克隆文库可以同时反映标本中的细菌种类和各种细菌的相对比例。本研究构建环境样品16S rRNA基因的克隆文库,解析长白山小天池入水口和长白瀑布下水潭两个典型水体样本中的细菌种属多样性特征,并探讨其与水体性质的关系。小天池文库共获得89条16S rRNA基因序列,分别属于10个类群,其中Betaproteobacteria是主要类群(占总数的23.6%)。长白瀑布文库共获得88条序列,分别属于8个类群,以Betaproteobacteria为主要类群(占总数的35.2%)。 BacteroidetesBetaproteobacteriaAlphaproteobacteriaGammaproteobacteria是两个样本共有的优势类群,而DeltaproteobacteriaAcidobacteriaArmatimonadetes只在小天池入水口处检出。长白瀑布水潭中的细菌群落多样性明显偏低,种群均匀度指数也较低,但两样本的地理距离极短,认为人类活动可能是导致这种差异的原因。

关键词 克隆文库   多样性   细菌群落结构   长白山  
Comparison of bacterial diversity based on typical water samples from Xiaotianchi lake and Changbai waterfall in Changbai Mountain
ZHANG Xiu-yuan1, MIAO Yu-yan2,3, GU Zheng-quan4, LIU Feng-xiang5
1. College of Mechanical Engingering, Nanjing University of Science and Technology, Nanjing 210094, China;
2. State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, China;
3. Beijing Museum of Natural History, Beijing 100050, China;
4. Institute of Tibetan Plateau Research Chinese Academy of Sciences, Beijing 100101, China;
5. The Geological Museum of China, Beijing 100034, China
Abstract:

Microorganisms in water can indicate changes in the environment with sensitive response. Through 16S rRNA clong library method, the authors can analyze the species of bacterial in samples, and know the relative proportion among all kinds of bacteria and etc. Therefore, this method has been widely applied to the environmental microbiology research. Through 16S rRNA clong library method, bacterial diversity characteristics and its relationship with the nature of waters from Xiaotianchi lake (water inletentry point) and Changbai waterfall (plunge pool) were analyzed. 89 gene sequences of 16S rRNA from Xiaotianchi lake were obtained, belonging to 10 groups. Among them, Betaproteobacteria is the major group dominating by 23.6%. 88 gene sequences of 16S rRNA from Changbai waterfall were obtained, belonging to 8 groups. Among them, Betaproteobacteria is the major group dominating by 35.2%. Bacteroidetes, Betaproteobacteria,Alphaproteobacteria and Gammaproteobacteria were the main bacteria groups in both of the two environments, while Deltaproteobacteria,Acidobacteria and Armatimonadetes were only detected from the water entry point of Xiaotianchi lake. Both the bacterial community diversity and species evenness index from the plunge pool of Changbai waterfall are lower than those from the water inlet of Xiaotianchi lake. The two sampling sites are not far apart with similar natural environment, but quite different on the level of human activities. The authors argue that human activities may be the cause of differences between both bacteria community diversity.

Keywords: clone library   diversity   bacterial community structure   Changbai Mountain  
收稿日期 2017-03-06 修回日期 2017-04-17 网络版发布日期  
DOI: 10.3969/j.issn.1004-5589.2017.02.031
基金项目:

中国地质大学生物地质与环境地质国家重点实验室开放基金项目(GBL21302).

通讯作者: 苗雨雁(1969),女,高级工程师,主要从事地层古生物学研究.E-mail:myyzzh@126.com
作者简介:
作者Email: myyzzh@126.com

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