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ˮ����΢����Ի����ĸı��������е���Ӧ��16S rRNA�����¡�Ŀ����ͬʱ��ӳ�걾�е�ϸ������͸���ϸ������Ա��������о�����������Ʒ16S rRNA����Ŀ�¡�Ŀ⣬��������ɽС�����ˮ�ںͳ����ٲ���ˮ̶��������ˮ�������е�ϸ��������������������̽������ˮ�����ʵĹ�ϵ��С����Ŀ⹲���89��16S rRNA�������У��ֱ�����10����Ⱥ������Betaproteobacteria����Ҫ��Ⱥ��ռ������23.6%���������ٲ��Ŀ⹲���88�����У��ֱ�����8����Ⱥ����BetaproteobacteriaΪ��Ҫ��Ⱥ��ռ������35.2%���� Bacteroidetes��Betaproteobacteria��Alphaproteobacteria��Gammaproteobacteria�������������е�������Ⱥ����Deltaproteobacteria��Acidobacteria��Armatimonadetesֻ��С�����ˮ�ڴ�����������ٲ�ˮ̶�е�ϸ��Ⱥ�����������ƫ�ͣ���Ⱥ���ȶ�ָ��Ҳ�ϵͣ����������ĵ�����뼫�̣���Ϊ���������ǵ������ֲ����ԭ��

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Comparison of bacterial diversity based on typical water samples from Xiaotianchi lake and Changbai waterfall in Changbai Mountain
ZHANG Xiu-yuan1, MIAO Yu-yan2,3, GU Zheng-quan4, LIU Feng-xiang5
1. College of Mechanical Engingering, Nanjing University of Science and Technology, Nanjing 210094, China;
2. State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, China;
3. Beijing Museum of Natural History, Beijing 100050, China;
4. Institute of Tibetan Plateau Research Chinese Academy of Sciences, Beijing 100101, China;
5. The Geological Museum of China, Beijing 100034, China
Abstract:

Microorganisms in water can indicate changes in the environment with sensitive response. Through 16S rRNA clong library method, the authors can analyze the species of bacterial in samples, and know the relative proportion among all kinds of bacteria and etc. Therefore, this method has been widely applied to the environmental microbiology research. Through 16S rRNA clong library method, bacterial diversity characteristics and its relationship with the nature of waters from Xiaotianchi lake (water inletentry point) and Changbai waterfall (plunge pool) were analyzed. 89 gene sequences of 16S rRNA from Xiaotianchi lake were obtained, belonging to 10 groups. Among them, Betaproteobacteria is the major group dominating by 23.6%. 88 gene sequences of 16S rRNA from Changbai waterfall were obtained, belonging to 8 groups. Among them, Betaproteobacteria is the major group dominating by 35.2%. Bacteroidetes, Betaproteobacteria,Alphaproteobacteria and Gammaproteobacteria were the main bacteria groups in both of the two environments, while Deltaproteobacteria,Acidobacteria and Armatimonadetes were only detected from the water entry point of Xiaotianchi lake. Both the bacterial community diversity and species evenness index from the plunge pool of Changbai waterfall are lower than those from the water inlet of Xiaotianchi lake. The two sampling sites are not far apart with similar natural environment, but quite different on the level of human activities. The authors argue that human activities may be the cause of differences between both bacteria community diversity.

Keywords: clone library   diversity   bacterial community structure   Changbai Mountain  
�ո����� 2017-03-06 �޻����� 2017-04-17 ����淢������  
DOI: 10.3969/j.issn.1004-5589.2017.02.031
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